Follicular Stimulating Hormone (FSH) a reproductive hormone that is produced by the pituitary gland in the brain. FSH stimulates the growth and development of unfertilised eggs during the menstrual cycle in women and initiates the production of sex hormones such as oestradiol and progesterone. FSH is also used to stimulate the production of sperm in men. The FSH hormone is often tested with other sex hormones such as LH, testosterone, oestradiol and progesterone for diagnose infertility and pituitary gland disorders in men and women.

 Oestradiol is a form of oestrogen and the main female hormone produced by non-pregnant women. The hormone has an important role in the development of female physical features and reproductive functions. Oestradiol tests are used for the evaluation of ovarian functions. It can also be used to diagnose the cause of precocious and delayed puberty in girls as well as used to monitor hormone replacement therapy in peri-pausal and menopausal women

Progesterone plays an important role in preparing a female body for pregnancy. This test can help to assess if a woman is ovulating normally and should be done on day 21 into a cycle.